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Overexpression of SmbHLH148 induced biosynthesis of tanshinones as well as phenolic acids in Salvia miltiorrhiza hairy roots
Bingcong Xing1,2; Lijun Liang1,2; Lin Liu4; Zhuoni Hou3; Dongfeng Yang3; Kaijing Yan5; Xuemin Zhang5; Zongsuo Liang1,3,4
2018-12-18
Source PublicationPlant Cell Reports
Volume12Issue:37Pages:1681–1692
Abstract

Phenolic acids and tanshinones are the two important groups of pharmaceutical ingredients presented in Salvia miltiorrhiza Bunge. The bHLH transcription factors could regulate secondary metabolism efficiently in plants. However, there are only some MYCs have been studied on regulation of either phenolic acids or tanshinones biosynthesis. In this study, a bHLH TF named SmbHLH148, which is homologous to AtbHLH148, AtbHLH147 and CubHLH1, was isolated and functionally characterized from S. miltiorrhiza. Transcription of SmbHLH148 could be intensely induced by ABA and also be moderately induced by MeJA and GA. SmbHLH148 is present in all the six tissues and mostly expressed in fibrous root and flowers. Subcellular localization analysis found that SmbHLH148 was localized in the nucleus. Overexpression of SmbHLH148 significantly increased not only three phenolic acids components accumulation but also three tanshinones content. Content of caffeic acid, rosmarinic acid and salvianolic acid B were reached to 2.87-, 4.00- and 5.99-fold of the control in the ObHLH148-3, respectively. Content of dihydrotanshinone I, cryptotanshinone, and tanshinone I were also present highest in ObHLH148-3, reached 2.5-, 5.04- and 3.97-fold of the control, respectively. Expression analysis of pathway genes of phenolic acids and tanshinones in transgenic lines showed that most of them were obviously upregulated. Moreover, transcription of AREB and JAZs were also induced in SmbHLH148 overexpression lines. These results suggested that SmbHLH148 might be taken part in ABA and MeJA signaling and activated almost the whole biosynthetic pathways of phenolic acids and tanshinones, thus the production of phenolic acids and tanshinones were upregulated.

KeywordBhlh Transcription Factor Phenolic Acids Tanshinones Transgenic Secondary Metabolism Salvia Miltiorrhiza
MOST Discipline Catalogue理学::生物学
DOI10.1007/s00299-018-2339-9
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Indexed BySCI
Language英语
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/8803
Collection水保所2018--2019届毕业生论文
Corresponding AuthorZongsuo Liang
Affiliation1.Institute of soil and water conservation, CAS and MWR
2.University of Chinese Academy of Sciences
3.College of Life Sciences, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University
4.College of Life Sciences, Northwest A&F University
5.Tasly R&D Institute, Tasly Holding Group Co. Ltd
Recommended Citation
GB/T 7714
Bingcong Xing,Lijun Liang,Lin Liu,et al. Overexpression of SmbHLH148 induced biosynthesis of tanshinones as well as phenolic acids in Salvia miltiorrhiza hairy roots[J]. Plant Cell Reports,2018,12(37):1681–1692.
APA Bingcong Xing.,Lijun Liang.,Lin Liu.,Zhuoni Hou.,Dongfeng Yang.,...&Zongsuo Liang.(2018).Overexpression of SmbHLH148 induced biosynthesis of tanshinones as well as phenolic acids in Salvia miltiorrhiza hairy roots.Plant Cell Reports,12(37),1681–1692.
MLA Bingcong Xing,et al."Overexpression of SmbHLH148 induced biosynthesis of tanshinones as well as phenolic acids in Salvia miltiorrhiza hairy roots".Plant Cell Reports 12.37(2018):1681–1692.
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