ISWC OpenIR  > 水保所知识产出(1956---)
短期盐胁迫对苦苣菜幼苗叶片 抗逆生理指标的影响
贾鹏燕1,2,3; 田福平2; 刘一帆1,3; 刘   玉1; 武高林1
2017
Source Publication西北植物学报
Volume37Issue:7Pages:西北植物学报
Abstract

采用蛭石中浇灌 Hoagland营养液的基质培养方法,以苗期苦苣菜为实验材料,设置5个 NaCl处理浓度( 0
( CK )、
66 、 133 、 200 、 250 、 300mmol · L
-1 ),分别在处理1 、
2 、 3d采集苦苣菜叶片,测定其 Na
+ 、
K + 、丙二醛( MDA )、
可溶性糖,可溶性蛋白质及游离脯氨酸含量,以及超氧化物歧化酶( SOD )、过氧化物酶( POD )和过氧化氢酶( CAT )
活性,探讨短期盐胁迫下苦苣菜叶片生理指标的变化特征,为进一步研究野生物种的耐盐性及耐盐机制提供理论
依据。结果表明:( 1 )盐胁迫 1d 和 2d 、
NaCl 浓度为 66~200mmol · L
-1 时,苦苣菜叶片
Na
+ 含量无显著变化,
K +
含量和 K + / Na
+ 较对照显著增大,并在 200mmol · L -1 NaCl 处理下达到最大值;盐胁迫 3d 时,叶片 K + 含量与胁
迫前 2d 的变化趋势相似, Na
+ 含量随盐浓度升高显著增大,
K + / Na
+ 除 66mmol · L -1 NaCl 处理外均随盐浓度升
高而显著减小。( 2 )盐胁迫 1~3d 时,苦苣菜叶片可溶性蛋白质、可溶性糖含量均随盐浓度升高而先增后减,可溶
性蛋白质含量在胁迫 1~2d 、可溶性糖在胁迫 2~3d 时均在 200mmol · L -1 NaCl 下达到最大值;游离脯氨酸含量
在盐胁迫前2d 时与可溶性蛋白质、可溶性糖的变化趋势相似,在胁迫 3d 时随盐浓度升高而显著增大且绝大部分
与对照差异显著。( 3 )苦苣菜叶片 SOD 、
POD 、 CAT 活性在盐胁迫 1~3d 时均随盐浓度升高而先增后减;在胁迫 1
~2d 时, 3 种酶活性均在 250mmol · L
-1 NaCl 下达到最大值且所有盐浓度下均显著大于对照;在盐胁迫 3d
时,
SOD 、 POD 活性在 200mmol · L
-1 NaCl 下达到最大值,
CAT 活性在 133mmol · L
-1 NaCl 下达到最大值且除 250 、
300mmol · L
-1 NaCl 外均与对照差异显著。研究发现,苦苣菜具有较强的耐盐性,能够在盐胁迫(
66~300mmol
· L -1 )处理 1~3d 内进行一系列有效的生理调节,增强自身渗透调节能力及抗氧化能力,表现出较强的吸钾拒钠
的特性,基本缓解了 Na
+ 的毒害及渗透压力,但是
NaCl 浓度超过 200mmol · L
-1 后其渗透调节能力和抗氧化能力
均显著降低。

Other Abstract

 In order to evaluate the responses of physiological indexes of Sonchus oleraceus to short - term salt
stress and provide theoretical instructions to the further research of salt tolerance and tolerance mechanism
for wild plants , we irrigated S . oleraceus seedings with Hoagland ’ s nutrient solution containing NaCl at
various concentrations [ 0 ( control ), 66 , 133 , 200 , 250 , 300mmol · L
-1 ]
.After 1 , 2and 3day of salt
treatments , the leaves of plants were harvested , Na
+ ,
K + , malondialdehyde ( MDA ), soluble sugar , sol -
uble protein , free proline contents and superoxide dismutase ( SOD ), peroxidase ( POD ) and catalase
( CAT ) activities were measured.The results showed that :(
1 ) after 1 , 2day of 66~200mmol · L
-1
NaCl , the changes of Na
+ content were not obvious.K + content and K + / Na + ratio significantly
increased
with salinity compared with CK and reached to the maximun at 200mmol
· L -1 NaCl.After 3day of salt
treatment , the changes of K + content had the similar tendency with 1day and 2day treatments.Na
+ con

tent significantly increased with salinity.K
+ / Na +
ratio significantly decreased with salinity
except at 66
mmol · L
-1 NaCl. (
2 ) Soluble sugar , soluble protein and proline contents increased , then decreased with
salinity after 1-3day
of salt stress.At 200mmol · L
-1 NaCl ,
soluble protein content reached to the max -
imun after 1day and 2day
salt stress and soluble sugar content reached to the maximun after 2day and 3
day
salt stress.Free proline had the similar tendency after 1day and 2day
salt stress and significantly in -
creased compared with CK with salinity after 3day salt stress. (
3 ) The activities of SOD , POD and CAT
increased then decreased with salinity after 1~3day
salt stress.All of them reached to the maximun at 250
mmol · L
-1 NaCl and were much higher than CK at all levels of salinity after 1day
and 2day salt stress.
The activities of SOD and POD reached to the maximun at 200mmol · L
-1 NaCl after 3day
salt stress ,
during which CAT activity
reached to the maximun at 250mmol · L
-1 NaCl and were much higher than CK
except at 250and 300mmol · L
-1 NaCl.It was demonstrated that S . oleraceus was very salt tolerant and
could tolerance 66-300mmol · L
-1 NaCl ,
as judged from the higher ability to absorb K

and reject Na

as well as the higher osmoregulation and antioxidant capacity , almost relieving the toxicity of Na
+ and os

motic stress.But , the osmoregulation and antioxidant capacity decrease when NaCl concentration exceed
200mmol · L
-1 .

Keyword苦苣菜 Nacl 短期胁迫 渗透调节物质 抗氧化酶
Indexed By中文核心期刊要目总览
Language中文
Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/8679
Collection水保所知识产出(1956---)
Affiliation1.西北农林科技大学 黄土高原土壤侵蚀与旱地农业国家重点实验室,陕西杨陵, 712100
2.中国农业科学院兰州畜牧与兽药研 究所/农业部兰州黄土高原生态环境重点野外科学观测试验站,兰州 730050
3.西北农林科技大学 林学院,陕西杨陵 712100
Recommended Citation
GB/T 7714
贾鹏燕,田福平,刘一帆,等. 短期盐胁迫对苦苣菜幼苗叶片 抗逆生理指标的影响[J]. 西北植物学报,2017,37(7):西北植物学报.
APA 贾鹏燕,田福平,刘一帆,刘   玉,&武高林.(2017).短期盐胁迫对苦苣菜幼苗叶片 抗逆生理指标的影响.西北植物学报,37(7),西北植物学报.
MLA 贾鹏燕,et al."短期盐胁迫对苦苣菜幼苗叶片 抗逆生理指标的影响".西北植物学报 37.7(2017):西北植物学报.
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