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长尖对齿藓原丝体快速培育的关键影响因子研究
赵洋1,2; 李晓明4; 李茹雪3; 王春3; 卜崇峰1,3
2017
Source Publication植物研究
Volume37Issue:2Pages:185-193
Abstract

为了给苔藓结皮野外恢复的大规模接种提供丰富种源,本文利用0. 1% NaClO(10、15、20、30、45、60、120
s)和0. 1% HgCl2
(10、15、20、30、45、60、120 s) 两种消毒液,采用Knop、MS 和Hoagland 3 种培养基,设置5. 5、6、
6. 5、7、7. 5、8、8. 5 七个水平的pH 值,通过单因素试验设计方法,测定长尖对齿藓[Didymodon ditrichoides(Broth.)]
茎叶体的成活率、原丝体长度和分枝数等指标,探讨影响长尖对齿藓原丝体扩繁的关键因子。结果表明:(1) 消毒
方式、培养基及pH 均对长尖对齿藓茎叶体的成活率、原丝体长度和分枝数有显著影响(P < 0. 05),影响大小依次
均为消毒方式> 培养基> pH;(2)最适合的消毒方式为:0. 1%NaClO 消毒15 ~ 20 s;(3)最适合长尖对齿藓原丝体
生长的培养基是Knop 和Hoagland 培养基;(4)最适合长尖对齿藓原丝体生长的pH 是7. 5。从而得出快速培育长
尖对齿藓原丝体的最佳组合为:0. 1% NaClO 消毒15 ~ 20 s + Hoagland /Knop + pH = 7. 5。本文的研究结果可以缩
短长尖对齿藓生长周期以进行快速繁殖,为苔藓结皮的快速培育以及工程化应用提供一定的借鉴。

Other Abstract

In order to provide a rich source of mass vaccination for the field restoration of moss crust,we used
two kinds of disinfection method,0. 1% NaClO(10,15,20,30,45,60,120 s) and 0. 1% HgCl2
(10,15,
20,30,45,60,120 s),with Knop,MS and Hoagland as culture media,and set the pH values(5. 5,6,6. 5,
7,7. 5,8,8. 5),through the single factor experiment design method,we determined the survival rate of the
cormus of [Didymodon ditrichoides(Broth. )],protonema length and number of branches,thereby exploring
key factors that affect the propagation of the protonema of D. ditrichoides. The results are as follows: (1) The
disinfection method,culture medium and pH value have significant influence upon the survival rate of the
cormus of D. ditrichoides,length of protonema and number of branches(P < 0. 05),in a descending order of
disinfection method,culture medium,and pH value; (2)The most suitable disinfection method is 0. 1% NaClO
with a duration of 15 - 20 s; (3) The most suitable culture medium for the growth of the protonema of D.
ditrichoides is Knop and Hoagland; (4)The most suitable pH value for the growth of protonema of D. ditrichoidesis 7. 5. Therefore,the best combination of factors for rapid cultivation of the protonema of D. ditrichoides is
0. 1% NaClO disinfection 15 - 20 s + Hoagland /Knop + pH = 7. 5. Our experiment can be determined to
shorten the growth cycle of D. ditrichoides and realize rapid propagation,thereby providing experience for the
rapid culture and engineering application of moss crust.

Keyword长尖对齿藓 原丝体 消毒方式 培养基 Ph
Indexed By中文核心期刊要目总览
Language中文
Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/8524
Collection水保所知识产出(1956---)
Affiliation1.中国科学院水利部水土保持研究所
2.中国科学院大学
3.西北农林科技大学水土保持研究所
4.西北农林科技大学资源与环境学院
First Author Affilication中国科学院水利部水土保持研究所
Recommended Citation
GB/T 7714
赵洋,李晓明,李茹雪,等. 长尖对齿藓原丝体快速培育的关键影响因子研究[J]. 植物研究,2017,37(2):185-193.
APA 赵洋,李晓明,李茹雪,王春,&卜崇峰.(2017).长尖对齿藓原丝体快速培育的关键影响因子研究.植物研究,37(2),185-193.
MLA 赵洋,et al."长尖对齿藓原丝体快速培育的关键影响因子研究".植物研究 37.2(2017):185-193.
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