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Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings
Juan Chen1,2; Yu-Qing Liu1; Xiao-Wu Yan4; Ge-Hong Wei4; Jian-Hua Zhang2,3; Lin-Chuan Fang1; Fang, LC (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China.
SubtypeArticle
2018
Source PublicationECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN0147-6513
description.correspondentemailflinc629@hotmail.com
Volume162Pages:312-323
AbstractDespite numerous reports that legume-rhizobium symbiosis alleviates Cu stress in plants, the possible roles of legume-rhizobium symbiosis and the regulatory mechanisms in counteracting Cu toxicity remain unclear. Here, Sinorhizobium meliloti CCNWSX0020 was used for analyzing the effects of rhizobium inoculation on plant growth in Medicago saliva seedlings under Cu stress. Our results showed that rhizobium inoculation alleviated Cu-induced growth inhibition, and increased nitrogen concentration in M. sativa seedlings. Moreover, the total amount of Cu uptake in inoculated plants was significantly increased compared with non-inoculated plants, and the increase in the roots was much higher than that in the shoots, thus decreasing the transfer coefficient and promoting Cu phytostabilization. Cu stress induced lipid peroxidation and reactive oxygen species production, but rhizobium inoculation reduced these components' accumulation through altering antioxidant enzyme activities and regulating ascorbate-glutathione cycles. Furthermore, legume-rhizobium symbiosis regulated the gene expression involved in antioxidant responses, phytochelatin (PC) biosynthesis, and metallothionein bio-synthesis in M. sativa seedlings under Cu stress. Our results demonstrate that rhizobium inoculation enhanced Cu tolerance by affecting Cu uptake, regulating antioxidant enzyme activities and the ascorbate-glutathione cycle, and influencing PC biosynthesis-related gene expression in M. sativa. The results provide an efficient strategy for phytoremediation of Cu-contaminated soils.
KeywordAntioxidant Enzymes Ascorbate-glutathione Cycle Copper Stress Legume-rhizobium Phytoremediation
Subject AreaEnvironmental Sciences & Ecology ; Toxicology
DOI10.1016/j.ecoenv.2018.07.001
Indexed BySCI
Publication Place525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
Language英语
WOS IDWOS:000440879500037
PublisherACADEMIC PRESS INC ELSEVIER SCIENCE
Funding OrganizationNational Key Research and Development Program of China [2016YFD0800706] ; National Key Research and Development Program of China [2016YFD0800706] ; National Natural Science Foundation of China (NSFC) [31501822, 41571314] ; National Natural Science Foundation of China (NSFC) [31501822, 41571314]
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Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/8180
Collection水保所科研产出--SCI_2018--SCI
Corresponding AuthorFang, LC (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China.
Affiliation1.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Fanning Loess P, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Univ Hong Kong, Sch Life Sci, Hong Kong, Hong Kong, Peoples R China
3.Chinese Univ Hong Kong, State Key Lab Agrobiotechnol, Hong Kong, Hong Kong, Peoples R China
4.Northwest A&F Univ, Sch Life Sci, Yangling 712100, Shaanxi, Peoples R China
5.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Juan Chen,Yu-Qing Liu,Xiao-Wu Yan,et al. Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2018,162:312-323.
APA Juan Chen.,Yu-Qing Liu.,Xiao-Wu Yan.,Ge-Hong Wei.,Jian-Hua Zhang.,...&Fang, LC .(2018).Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,162,312-323.
MLA Juan Chen,et al."Rhizobium inoculation enhances copper tolerance by affecting copper uptake and regulating the ascorbate-glutathione cycle and phytochelatin biosynthesis-related gene expression in Medicago sativa seedlings".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 162(2018):312-323.
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