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Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.
Ru, Mei1,2; Wang, Kunru3; Bai, Zhenqing3; Peng, Liang4; He, Shaoxuan5; Pei, Tianlin3; Jia, Yanyan3; Li, Hongyan3; Liang, Zongsuo1,2; Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China.
SubtypeArticle
2017
Source PublicationPLANT CELL TISSUE AND ORGAN CULTURE
ISSN0167-6857
description.correspondentemailliangzs@ms.iswc.ac.cn
Volume128Issue:2Pages:381-390
AbstractPrunella vulgaris, a widely-used medicinal perennial herb, contains various active compounds and has multifaceted medicinal activities. As the index component in P. vulgaris, rosmarinic acid (RA) is a typical phenolic acid, with significant anti-inflammatory, anti-oxidant, anti-tumour, anti-viral, and anti-microbial properties. To better understand the RA biosynthetic pathway in P. vulgaris, we isolated and cloned the cDNA sequences of putative RA synthase (PvRAS) and cytochrome P450 monooxygenase (PvCYP98A101), two important enzymes catalysing RA biosynthesis. Sequence analysis revealed that PvRAS contained an open reading frame (ORF) of 1305 bp encoding a 435-amino-acid residue belonging to the BAHD acyltransferase family, and PvCYP98A101 contained an ORF of 1530 bp encoding a 510-amino-acid residue, as a member of the CYP98A family. The deduced PvRAS and PvCYP98A101 amino acid sequences shared high similarity with other putative/known RASs and CYPs, respectively. Quantitative real-time PCR analysis showed that constitutive expression of PvRAS and PvCYP98A101 was much higher in roots than in leaves, stems, or spikes. Further analysis indicated that PvRAS was localised in the cytosol and nucleus, whilst PvCYP98A101 existed as a membrane protein in the endoplasmic reticulum. These results highlight the RA biosynthesis pathway in P. vulgaris and, provide useful information to engineer natural products.
KeywordPrunella Vulgaris Rosmarinic Acid Rosmarinic Acid Synthase Cytochrome P450 Monooxygenase Subcellular Localisation Expression Pattern
Subject AreaBiotechnology & Applied Microbiology ; Plant Sciences
DOI10.1007/s11240-016-1117-z
URL查看原文
Indexed BySCI
Publication PlaceDORDRECHT
Language英语
WOS IDWOS:000393748500013
PublisherSPRINGER
Funding OrganizationTwelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; Doctoral Fund of Ministry of Education of China [20110204110028]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; Doctoral Fund of Ministry of Education of China [20110204110028]; National Natural Science Foundation of China [81373908]
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Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/8040
Collection水保所科研产出--SCI_2017--SCI
Corresponding AuthorLiang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Chinese Acad Sci, Minist Water Resources, Yangling 712100, Peoples R China
3.Northwest A&F Univ, Coll Life Sci, Yangling, Peoples R China
4.Shaanxi Univ Chinese Med, Coll Pharm, Xian, Peoples R China
5.Environm Protect Agcy, Environm Monitoring Stn, Chongqing, Peoples R China
Recommended Citation
GB/T 7714
Ru, Mei,Wang, Kunru,Bai, Zhenqing,et al. Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2017,128(2):381-390.
APA Ru, Mei.,Wang, Kunru.,Bai, Zhenqing.,Peng, Liang.,He, Shaoxuan.,...&Liang, ZS .(2017).Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L..PLANT CELL TISSUE AND ORGAN CULTURE,128(2),381-390.
MLA Ru, Mei,et al."Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L.".PLANT CELL TISSUE AND ORGAN CULTURE 128.2(2017):381-390.
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