ISWC OpenIR  > 水保所科研产出--SCI  > 2017--SCI
A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L
Ru, Mei1,2; Wang, Kunru1,2; Bai, Zhenqing1,2; Peng, Liang3; He, Shaoxuan4; Wang, Yong1,2; Liang, Zongsuo1,2; Liang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.
SubtypeArticle
2017
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
description.correspondentemailliangzs@ms.iswc.ac.cn
Volume7
AbstractRosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K-m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate. In vivo activity was confirmed by functional restoration of the Escherichia coli tyrosine auxotrophic mutant DL39. Agrobacterium rhizogenes-mediated antisense/sense expression of PvTAT in hairy roots was used to evaluate the contribution of PvTAT to RA synthesis. PvTAT were reduced by 46-95% and RA were decreased by 36-91% with low catalytic activity in antisense transgenic hairy root lines; furthermore, PvTAT were increased 0.77-2.6- fold with increased 1.3-1.8-fold RA and strong catalytic activity in sense transgenic hairy root lines compared with wild-type counterparts. The comprehensive physiological and catalytic evidence fills in the gap in RA-producing plants which didn't provide evidence for TAT expression and catalytic activities in vitro and in vivo. That also highlights RA biosynthesis pathway in P. vulgaris and provides useful information to engineer natural products.
Subject AreaScience & Technology - Other Topics
DOI10.1038/s41598-017-05290-4
URL查看原文
Indexed BySCI
Publication PlaceLONDON
Language英语
WOS IDWOS:000404970900054
PublisherNATURE PUBLISHING GROUP
Funding OrganizationTwelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908] ; Twelfth Five-Year Plan for Science & Technology Support of China [2015BAC01B03]; National Natural Science Foundation of China [81373908]
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Document Type期刊论文
Identifierhttp://ir.iswc.ac.cn/handle/361005/7961
Collection水保所科研产出--SCI_2017--SCI
Corresponding AuthorLiang, ZS (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Minist Water Resources, Yangling 712100, Peoples R China.; Liang, ZS (reprint author), Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Soil & Water Conservat, Yangling 712100, Peoples R China
2.Minist Water Resources, Yangling 712100, Peoples R China
3.Shannxi Univ Chinese Med, Coll Pharm, Xian 710000, Peoples R China
4.Environm Protect Agcy, Ecol Environm Monitoring Stn, Dazu 402360, Peoples R China
5.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310000, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Ru, Mei,Wang, Kunru,Bai, Zhenqing,et al. A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L[J]. SCIENTIFIC REPORTS,2017,7.
APA Ru, Mei.,Wang, Kunru.,Bai, Zhenqing.,Peng, Liang.,He, Shaoxuan.,...&Liang, ZS .(2017).A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L.SCIENTIFIC REPORTS,7.
MLA Ru, Mei,et al."A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L".SCIENTIFIC REPORTS 7(2017).
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